Manufacturers of ethanol often have the goal of increasing their production of alcohol while simultaneously lowering their costs. This ideal outcome may be achieved by using the dual enzymes ethanol production technique with boli brand thermostable alpha-amylase for liquefaction and glucoamylase for saccarification in the medium-temperature cooking process that is associated with the manufacture of ethanol. What might cause this to occur? Permit me to provide you with an in-depth look at the procedure. Instead, we need to have the following items ready before we begin, which are as follows: The thermostable alpha-amylase boli ha-320 belongs to the enzyme ethanol fermentation (a red liquid with an enzyme activity of 40,000 u/ml), the glucoamylase boli ga-130 (a light to brown lightly liquid with an enzyme activity of 150,000 u/ml), they are resistant to high temperatures, and they are highly active dry yeast. When these resources are available, the process begins with determining whether or not corns are available, and then it continues with the elimination of any undesired alien elements. The corn is processed in a hammer mill, which grinds it into flour or powder that exposes the cornstarch. After that, the corn is sieved into qualifying corn powders. This procedure occurs after the corn has been selected. In phase 2, we could find it feasible to use: Enzymes are fuel for ethanol. Within a slurry tank is where the mixing process takes place. It is possible to weigh the corn powders before they are poured into the tank. This is done so that the appropriate amount of water may be added to the mixture. Centrifuged liquid that is connected with the distiller’s grain should be fed into this tank at a rate of thirty percent to forty percent recycling. At the same time, thirty percent ammonia or naoh solution should be introduced all at once. This is accomplished with the intention of maintaining the ph value inside the liquefaction tank at a range of 5.6 to 6.2. In order to liquefy one meter of maize material, it is necessary to add enzymes that produce ethanol at a dose level that falls between 0.42 and 0.80 kg. When the mixing process is over, we may simply go on to the following phase, which is the liquefaction of the mixture.